The French player and Hungarian partner Timea Babos defended their Roland Garros doubles title on Sunday, winning their fourth Grand Slam title together.
She was placed in quarantine and the U.S. Tennis Association removed the top-seeded team of Mladenovic and Babos from the doubles competition.
Seeded second in Paris, Babos and Mladenovic beat the 14th-seeded pairing of Alexa Guarachi from Chile and American Desirae Krawczyk 6-4, 7-5 in Sunday's final.
As well as winning at Roland Garros last year, they have also won twice at the Australian Open, in 2018 and 2020.
As well as four titles won with Babos, Mladenovic also won in the doubles at Roland Garros in 2016 with French countrywoman Caroline Garcia.